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ON THE ROAD TO EXCELLENCE IN UNRAVELLING THE (EPI)GENETIC LANDSCAPE OF HEMATOLOGIC NEOPLASMS

Online lecture on Hodgkin lymphoma

The lecture entitled ‘Unusual expression pattern of the negative NF-ĸB regulator miR-181a and its role in classical Hodgkin lymphoma’ took place.

The lecture was delivered by professor Maciej Giefing from the Department of Cancer Genetics of IHG PAS. The Lecture covered issues such as: classification of lymphomas, genetics of lymphomas,alterations observed in cHL, subtypes of DLBCL, miR-181a in activated B-cell like DLBCL, MiRNA expression and other.

Abstract: We aim to clarify the expression pattern of miRNA-181a in classical Hodgkin lymphoma (cHL) which is encoded by two host genes on chromosomes 1 and 9. By using discriminating real-time qPCR primers we found that cHL cell lines express only the miRNA-181a host gene located on chromosome 1 but not the one on chromosome 9. In consequence, cHL cell lines are characteristic for an attenuated expression of miR-181a-5p in contrast to the majority of non-Hodgkin lymphoma cell lines and all GCB cell polls analyzed, which showed stable expression of the miRNA. Moreover, using bisulfite pyrosequencing we demonstrated DNA hypermethylation of the promoter region of the host gene located on chromosome 9 is responsible for the observed downregulation. Last, in line with recent findings in ABC-like DLBCL, we demonstrate that induced overexpression of miRNA-181a in cHL presents a growth disadvantage for the tumor cells confirming its tumor suppressive role.